Syllabus of DMLT

MAHARASHTRA STATE BOARD OF VOCATIONAL EDUCATION EXAMINATION, MUMBAI

1		Name of Course		Diploma Course in Medical Lab Technician (W.E.F. 2015-16)
2		Course Code		201404
3		Max.No.of Students Per  Batch		25  Students
4		Duration		2 year
5		Type		Full Time
6		No.Of Days / Week		6 days
7		No.Of  Hours /Days		7 Hrs
8		Space Required		Theory Class Room –  200 sqft , Lab  Sub. –  400 sqft, Lab Elective - 400 Sq.ft.         =     Total    1000  Sq.ft.     +    MOU with Pathology Lab is required. Distance between Pathology Lab and Institute Should not be more  than 10 Km.
9		Minimum Entry Qualification		S.S.C. Pass
10		Objective Of  Course		To carry out medical laboratory technical work in various departmens in medical and pharmacy  colleges, peripheral laboratories, research and diagnostic centres, to setup and run own clinical laboratory.
11		Employment opportunities		He will assist the qualified person.
12		Teachers Qualification		For Vocational Subject - MBBS, OR  MBS Hom/BHMS, OR B. Pharmacy, OR M.Sc. Microbiology/Biochemistry/MLT + 2year Experience or Equivalent and for Non Vocational Subject Master Degree in concern Subject.
13]  Teaching Scheme –
	Sr.no					Subject  Code			Clock Hours / Week				Total
									Theory		Practical
	1		English (Communicatio n Skill)			90000001			2 Hrs		1 Hrs		3 Hrs
	2		Elective – I						2 Hrs		1 Hrs		3 Hrs
	3		Elective – II						2 Hrs		1 Hrs		3 Hrs
	4		Anatomy, Physiology a nd Pathology			20140004			3 Hrs		8 Hrs		11 Hrs
	5		Biochemistry			20140005			3 Hrs		8 Hrs		11 Hrs
	6		Microbiology and Medi cal care			20140006			3 Hrs		8 Hrs		11 Hrs
	Total												42 Hrs
14		Internship		Two Month Summer Internship from 1st May to 30th June is Compulsory.
15] E xamination Scheme – Final Examination will be based on syllabus of both years.
	Sr.no		Subject	Subject Code	Theory					P ractical				Total
					Duration	Max		Min	Duration		Max	Min		Max	Min
	1		English  (Communication Skill)	90000001	3 Hrs	70		25	3 Hrs		30	15		100	40
	2		Elective – I		3 Hrs	70		25	3 Hrs		30	15		100	40
	3		Elective – II		3 Hrs	70		25	3 Hrs		30	15		100	40
	4		Anatomy, Physiology and Pathology	20140004	3 Hrs	100		35	3 Hrs		100	50		200	85
	5		Biochemistry	20140005	3 Hrs	100		35	3 Hrs		100	50		200	85
	6		Microbiology and Medical care	20140006	3 Hrs	100		35	3 Hrs		100	50		200	85
	Total													900	375
16		Teachers – Three Teachers per batch for vocational component. For English, Elective-I & II guest faculty  on clock hour basis.
17		Student have to choose any one subject for Elective-I and Elective-II from below given subjects
18		a) For Elective I – Student can choose any one subject Code                        Subject Name 90000011    Applied  Mathematics 90000012    Business Economics 90000013    Physical Biology (Botany  &  Zoology) 90000014    Entrepreneurship 90000015    Psychology					b) For Elective II  – Student can choose any one                                      subject       Code                        Subject Name 90000021                    Applied Sciences(Physics & Chemistry) 90000022                    Computer Application 90000023  Business Mathematics

Theory  -   I   -   Anatomy, Physiology and Pathology – 1^st year

(Subject Code –   20140004)

 

Theory

Practical

ANATOMY PHYSIOLOGY Detailed Syllabus I. Basics in Anatomy 1.                  Introduction to Human Anatomy 2.                  Cell structure, properties of cell, tissues - epithelial, connective muscular, nervous 3.                  Digestive System and Hepato Biliary System 4.                  Respiratory System 5.                  Cardio vascular System 6.                  Lymphatic System 7.                  Bones and Joints 8.                  Nervous System 9.                  Endocrine System 10.              Sense Organs Eye, ear, skin, nose, tongue 11.              Excretory System 12.              Reproductive System Basics 1.      Introduction to Human Physiology 2.      Blood 3.      Cardio vascular system 4.      Lymphoid System 5.      Digestive system 6.      Respiratory system 7.      Nervous system 8.      Endocrine system 9.      Excretory System 10.  Reproductive system 11.  Sense organs

ANATOMY AND PHYSIOLOGY 1. Human Skeleton It includes - 1) Names of the Bones 2) Identification points 3) Surfaces (Skull, scapula, clavicle, humerus, radius, ulna, carpal bones, meta carpal bones, Phalanges. Innominate bone, Femur, patella tibia, fibula, tarsal bones, meta tarsal bones, Phalanges, Ribs-classification, vetebrae pieces, sternum.) 2.                  Human Organs Brain, Stomach  Lungs, Intestines Heart, Kidney Liver, Uterus Spleen, Fallopian tubes 3.                  Human slides Epithelial Tissue Connective Tissue Muscular Tissue Nervous Tissue Liver Kidney Spleen Pancreas Lymphnodes Skin testes Ovary Uterus Tonsil Stomach layers Small Intestine Large Intestine 4.                  Blood Pressure Estimation 5.                  T.P.R. (Temperature, pulse, respiration) Chart 6.                  TC, DLC, (TC - Total count RBC Total count of WBC DLC differential count of Leucocyts)

 

Anatomy, Physiology and Pathology   –    2^nd year

Theory	Practical
PATHOLOGY 1.                  Urine - Analysis - Physical Examination - specific gravity PH, reaction, colour Chemical Examination - Sugar Albumin, bile salts, bile Pigments etc. Microscopic Sediment for RBC, WBC, Epitheleal cells, casts, crystals, parasites Preparation of Reagents, procedure and principle of tests 2.                  Sputum Analysis - Physical Examination, Preparation and staining smear for Microscopic Examination 3.                  Semen Analysis- Physical Examination Microscopy - counting, motility, staining, Morphology, abnormal and normal forms. 4.                  Body Fluids - Differential count of Peritoneal, pericardial, pleural fluids and CSF, charging chamber, Identifying and counting the cells. 3. Haematology - a.  Collection of Blood - Methods of collection veinpuncture, finger puncture and vacutainer methods, materials required procedures, precautions, uses of the sample and advantages of each methods. POCT (sample collection at bed side) b.  Preparation of anti coagulants -	PATHOLOGY Blood Collection Precaution and smearing techniques and labelling of the sample Preparation of anticoagulants RBC, WBC, & platelet count ESR stands & ESR estimation PCV & calculation of RBC indices Hb estimation by different methods Urine - Physical Examination & Chemical Examination   PRACTICAL I.     Automatic Tissue Processer Microtome & Knives Centrifuge Hot air overn & Incubator Busm beaker, stop watch Glass Makers Simple balance & colorimeter Water bath - for tissue flotation Knowledge Maintenance & cleaning Care about tissue equipment II.   Maintenance & preservation of Cytology slides M.P. blocks & slides Histopathology specimens and process
Double oxalate, sodium citrate, EDTA, Heparin, action of each preparation, uses disadvantages, quantity required. c.  RBC, WBC Count : Methods (Microdilution and bulk dilution) Materials required, diluting fluids, preparation, procedures, advantages of each methods, precautuions, formula for calculation and clinical significance. d.  Platelet count : Morphology and functions of platelets diluting fluids, procedure, formula for calculation and clinical significances e.  Reticulocyte Count : Methods (dry & wet) staining, diluting fluids, normal Morphology and values, clinical significance. f.   Haemoglobin Estimation - Materials, procedure, of Tallquist, sahlis, Alkali haldanis, cyanmeth aemoglobin and S.G. method, advantages and disadvantaes and clinical significance g.  Estimation of PCV - Macro & Micro Method, procedure filling the tube, centrifuging and reading, advantages of each - normal values and clinical significance Estimation of Erythrocyte indices - calculation and importance MCV, MCH, MCHC, RDW, color index.	Preparation of form section material III.Glass ware Microslides & corverstips Sample collection bottles Micropathology Cytology. IV.              Immuno Haematology & blood banking ABO blood grouping techniques RH Factor Coombs test - Direct & indirect methods 34 V.                 Histopathology 1.  Fixation of biopsy tissue 2.  Processing of tissue Fixation Dehydration Clearing Impregnation Mounting Decalcification 3.  Mounting of museum specimens VI.      Cytology Fixations used Fluid preparation for cytological exam Slide preparation and staining Pap staining Mounting and preservation VII.   Sickle Cell Preparation VIII.Bone Marrow Smears Preparation
h. ESR - Methods used, procedure, stages, factors affecting and clinical significance I. Preparation of Blood smear examination - Making ideal films - slide method, cover glass method and staining, Morphology of RBC, WBC, Platelets and Haemop araasites. Differential Leucocyte Count - counting and identification of cells - Normal values, Morphology, procedure for smears and staining clinical significance and limitation. Absolute Eosinophil count - Materials, diluting fluid, procedure, identifying and counting the cells. II.                 Special stains on peripheral blood smear and bonemarrow smears- Ramanoskys stains, Leishman, Gemsia, wrights, Mycloperoxidase stain, PAS (Periodic Acid Schiff) - Preparation, method and selection of stain, Buffer Solution. Bone Marrow Smear - Preparing smears, cuithont crush artefacts staining and clinical significane. III.               Identification of Hemoparasites - Malarial Parasite, Microfilaria, Leishman making thick and thin films procedure and identification of parasite. IV.              Sickle Cell Preparation - Principle, procedure and Methods, Materials, clinical significance	& Staining IX. Cougulation Test, BT, CT.
V.                 Osmotic fragility test - Methods used, materials procedure, observation reporting, normal values, factors affecting, interpretation. VI.              Coagulation Tests a)                  Bleeding time - methods, dukes, Ivy's procedure Normal value, clinical significance b)                  Cloting time - methods, Lee & White, procedure materials, normal values, factors affecting coagulation clinical significance c)                  Prothrombin time (PT) d)                  APTT - in Detail VII.            L.E. cell Test - Principle, procedure, materials reproting, clinical significance and titration. Buffy coat preparation - LE Cell Test, Microfilaria Abnormal cells. VIII.         Basics of coulter counter IX.              Autopsy - Aims & methods of performing Autopsy cleaning, suturing and retaning the body. Cleaning autopsy instruments, tables and rooms, preservation of organs. Processing and preparation of Histopathology. X.                 Histopathology - 1) Biopsy 2) Processing of tissue a)  Fixation b)  Dehydration c)  Clearing
d)  Impregnation e)  Mounting f)   Declacification of Bone g)  Routine Paraffin staining h)  Immuno histochemists 3. Microtomes and Knifes XI.    Musuem Techniques Labelling & storage of specimens Methods of color maintenance Presentation of specimen Mounting labelling and cataloging the specimen Maintenance and cleaniness of the Museum Disposal of waste, safety in the lab XII.  Immuno Haematology and Blood Banking i) Introduction 2)                  Human blood group antigens, their inheritance and antihodies 3)                  ABO Blood group systems 4)                  RH Blood group system 5)                  Techniques of grouping and cross matching 6)                  Blood collection, Preservation and maintaining of Records 7)                  Coombs Test - a ) direct b) indirect

 

 

Biochemistry  -   1^st year

(Subject Code –   20140005 )

Theory	Practical
1.                  Introduction to Bio-chemistry including code of ethics for Medical Lab technicians and Medical Lab Organisation. 2.                  Reception, Registration and biochemical parameters investigated 3.                  Glassware and plastic ware used in a bio-chemical laboratory I. Glass ware : a)                  Types of glass and composition b)                  Types of glassware used, their identifiication, application and uses c)                  Cleaning, drying, maintenance and storage of glassware II. Plastic ware : Brief outline 4. Instrumental methods of Bio-chemical analysis : I.    Colorimetry : Visual and photoelectric methods, instrumentation, principles and laws involved, construction, operation, care and maintenance, applications II.  Spectrophotometry : Principle and theory, types, construction, and applications 5. Basic lab operations like - I. Separation of Solids from liquids, a) Centrifugation : Principle, Different types of Centrifges care and	1.                  Reception and registration 2.                  Collection of Capillaryblood 3.                  Collection of Venous blood 4.                  Collection of arterial blood 5.                  Separation of Serum from clotted blood 6.                  Separation of plasma from blood 7.                  Preparation of protein free blood filtrate 8. Lab glass ware a)  Identification b)  Handling c)  Care and Maintenance d)  Uses 9. Lab instruments a)  Centrifuges b)  Balances c)  Photo Electric colorimeter d)  Spectrophotometer 10. Preparation of a)  Percentage solutions b)  Normal solutions c)  Molar solutions 11.              Qualitative identification tests of sugars 12.              Qualitative identification tests of proteins 13.              Qualitative identification tests for amino acids 14.              Quantitative determination of Blood
maintenance, applications b) Filtration using funnel II.                 Weighing : Different types of balances used, care and maintenance. III.               Evaporation : IV) Distillation V) Refluxing VI) Drying different salts and dessicotion 6. Water, Chemicals and related substances I.       Purity of Chemicals II.    Corrosives. 8 III.  Hygroscopic Substances 7.                  Prevention, Safety and first aid in lab accidents. 8.                  Collection of specimens I. Blood : Types of Specimens, Collection, Precautions during collection, Processing and preservation. II. Urine : Types of Specimens, Collection of 24 hours urine and preservation 9.                  Urine biochemical parameters. 10.              Units of measurements 11.              Solutions Types based on solute and solvent, Types based on method of expressing concentration, calculations 12.              Carbohydrates and lipids I. Carbohydrates : Definition, Biological importance, classification, qualitative tests	sugar 15.              Glucose Tolerance test 16.              Quantitative determination of Blood urea 16.              Quantitative determination of Serum creatinine 17.              Quantitative determination of Urine Sugar.
II. Lipids : Definition, Biological importance, Classification, Acid value, Iodine value, saponification value. 13.              Amino acids and Proteins Definition, Biological importance, Classification, Qualitative tests. 14.              Diagnostic tests Blood Sugar, Glucose tolerance test, Blood urea, Serum uric acid, Serum creatinine. 15.              Vitamins and Minerals I. Vitamins : Water Soluble vitamins, Fat Soluble Vitamins, Sources, Daily requirements, Deficiency diseases II. Minerals : Sources, Daily requirements, Deficiency diseases

 

 

 

 

 

 

 

 

 

Biochemistry- 2^nd year

Theory	Practical
I. Instrumental methods of Bio-chemical analysis 1) Flame photometry : Principle, Theory, Construction of Flame Photometer, General and Clinical applications study of electrolytes using Flame photometer, Clinical importance of determination of electrolytes. 2.                  Fluorimetry : Fluorescence, Principle and theory, construction of Fluorimeter, General and Clinical applications 3.                  Nephelometry : Basic principle, instrumentation, general technique and clinical applications of Nephelometry. 4.                  Basic principles and application of Potentiometry II. Separation Techniques 1) Chromatography : Definition, Basic principles, different types and their techniques, General and clinical applications 2. Electrophoresis : Definition and basic principle involved, different types, instrumentation, general and clinical applications, electrophoretic fractionation of serum proteins and lipo proteins. III. Immuno assays - Definition, Basic Principles of immuno chemical reactions and immuno assays 1) Radio immuno assays Introduction to radio activity, Radio Pharmaceuticals, Safety and precautions, Hormone assays,	1)                  Electrophoretic fractionation of serum proteins and lipo proteins. 2)                  Separation of amino acids and carbohydrates by paper chromatography. 3)                  Determination of plasma prothrombin time 4)                  Oral glucose tolerance test 5)                  Estimation of serum calcium and inorganic phosphate 6)                  Practice and use of automated pipettes 7)                  Turbidimetric method of determination of plasma fibrinogen 8)                  Estimation of HDL cholesterol 9)                  Determination of Urinary 17 ketosteroids and VMA 10)              Determination of CPK, LDH, GGT and G6PD activities 11)              Determination of urine proteins by turbidimetric method 12)              CSF analysis - Pandy's test, Nonne - Apelt 13)              Demonstration of working of Auto analysers 14)              Training of Computer basics 15)              Estimation of serum sodium and potassium by Flame photometry 16)              Estimation of serum bicarbonate by titrimetric method. 17)              Demonstration of RIA
2) Enzyme linked immuno essays, Description of instruments used in these essays. IV. Metabolism : 1)                  Carbohydratemetabolism - Glycolysis and TCA Cycle, 2)                  Lipid metobolism - P - Oxidation of Fatty acids 3)                  Protein metabolism - Urea cycle 19 V.                 Titrimetric methods of Quantitative determination, preparation of various solutions used in titrimetric analysis. VI.              Liver function tests : 1)                  Basic concepts including normal and abnormal bilirubin metabolism 2)                  Classification 3)                  Serum bilirubin determination 4)                  Vandenbergh test 5)                  Total proteins and A/G ratio 6) Enzyme estimations as LFT. VII. Renal function tests : 1)  Basic concepts and classification 2)  Clearance tests 3)  Concentration and dilution tests and 4)  Urine examination in assessing kidney function VIII. Gastric function tests : 1)                  Basic concepts and introduction 2)                  Techniques of different tests including tubeless gastric analysis IX. Thyroid function tests : 1)  Basic Concepts 2)  Estimations of various thyroid hormones, their	18)              Demonstration of enzyme linked immuno assays 19)              Qualitative identification of urine sugars 20)              Qualitative identification of urine proteins 21)              Qualitative identification of urine BS & BP 22)              Gastric juice analysis, demonstration of stimulation tests 22 23)              Renal Calculi analysis 24)              Biliary Calculi analysis 25)              Demonstration of Ion selective electrodes 26)              Practice of Liver functions tests, and interpretation 27)              Practice of Renal functions tests, and interpretations 28)              Practice of Thyroid function tests and interpretations 29)              Practice and interpretation of cardiac profile and lipid profile 30)              Practice of quality control measures
interpretations 3) Recent methods of thyroid function tests X. Pancreatic function tests 1)                  Basic concepts and introduction 2)                  Various tests done and methods including serum amylase determination XI. Clinical Enzymology : 1)                  Introduction and Basic Concepts of Enzymes, Coenzymes and Isoenzymes 20 2)                  Importance of Enzymes 3)                  Transaminases 4)                  Cardiac Enzymes 5)                  Acid Phosphatase 6)                  Alkaline Phosphatase XII. Body Fluids : 1) Outlines of formations of different body fluids 2) Composition & analysis of CSF including a)                  CSF Sugar estimation b)                  CSF Proteins estimation c)                  CSF Chlorides estimation including interpretation of results XIII. Automation and usage of computers in Biochemical Analysis XiV. Quality assurance in Bio - Chemical laboratory. a)  Introduction and importance of quality assurance, General principle b)  Internal and external quality control
XV. Diagnostic tests a)  Lipid profile - Serum cholesterol, HDL Cholesterol b)  Glycosylated haemoglobin c)  Serum Calcium d)  Inorganic Phosphate e)  Analysis of hormone metabolites f)   Blood gas analysis

 

 

Microbiology and Medical care – 1^st year 

(Subject Code –   20140006 )

Theory	Practical
MICROBIOLOGY  I. Historical introduction to Microbiology contribution of famous Scientist in the field of Microbiology in brief a)  Anatony Van Lee wen Hook b)  Robert Koch c)  Edward Jenner d)  Louis Pasteur e)  Joseph Lister II. Microscopy a)                  Pinciple working and maintainance of compound Microscope b)                  Principle of Flourescent Microscope, Electron Microscope, Dark Ground Microscope III. Sterlization and disinfection - classification and Methods of sterilization 1) Principle and Methods of sterilization by heat a)                  By Dry Heat, Flamming, Red Heat, Hot air oven, incineration b)                  By Merit Heat-pasteurization, Inspissation, tyndalisation, autoclave 2)                  Filtration Methods 3)                  Ionising Radiation - Disinfection, Mode of action and uses of important chemical disinfections - Phenol and Phenolic compounds,	MICROBILOGY Lab Instructions for Personal Safety precaution Receipt and recording a specimen in the lab and dispatch of specimen Cleaning and care of glassware, syringes, apparatus, preparation of pasteur Pipettes. Handling and care of Microscope Operation of Autoclave, Incubator, waterbath, seitzfilter Preparation of various Media Preparation of stains and smears Methods of collection of microbidogy specimen-and its importance and processsing
alcohols, halogens, dyes and acids and alkalies 4) Gaseous Methods of sterilization. IV.              Cleaning, drying & Sterilization of Glassware disposal of contaminated material i.e. clinical infective material inoculated culture media. Handling and Disposal of Biomedical waste. V.                 Morphology and classification of Bacteria Sp. of cell, capsule, flagella, spore, Anaerobic Methods of cultivation of Bacteria.   VI.              Methods of Collection of clinical specimen for Micro-Biological investigation (indetail) like sputum- pettroff'smethod of concentration, urine, swabs, stool, blood, CSF and aspirations VII.            Processing of clinical specimen collected for Isolation and identification of organism a)                  Preparation of direct smear and staining b)                  Different Techniques of inoculation for isolation of bacteria c)                  Hanging drop preparation and its use d)                  Inoculation of various media for Biochemical reactions VIII. Compostion and preparation of staining reagents and different methods of staining a)  simple staining b)  Gram Staining c)  Spore staining
d)  Capsular staining e)  Zeihl Neelson staining f)   Albert staining g)  Negative staining h)  Flagellar staining i)    Flourescent staining IX. Culture Media - Classification of Media composition and preparation and uses. a)                  Basal Media - Peptone water, Nutrient broth, glucose broth. b)                  Enriched Media - Blood agar, Loefflers serum slope, chocolateagar c)                  Enrichment Media - Selenites broth, tetrathionatebroth Alkaline peptone water d)                  Differential Media - Maconkeys Media e)                  Selective Media - Lowenstcin Jenson Media, Potassium tellurite Media, TCBS, Wilson and Blair Media Deoxycholate citrate agar media   f)                   Blood culture media - Glucose broth, Hartleys broth, bile broth Sugar Media for Bio-chemical Reaction. Robertson cooked Meat Media, Thioglycolate media, Media and Reagents for differents Biochemical reaction i.e. Indole test, V.P. tests, M.R. test, citiate, urease, triple sugar Iron agar, Oxidase, catalase test, Nitrate reduction test, Pheny alkaline deaminase
test, glucose phosphate broth, gelatin liquifaction. Sabourauds dextrose Agar, PDA.

 

 

Microbiology and Medical care – 2^nd year 

Theory	Practical
I.                   Normal Flora of Micro-organisms in the Human Body. II.                Introduction of Iummunology - a)                  Brief outline of Immunity b)                  What are antigens c)                  What are antibodies d)                  Different-types of antigen and antibody reaction their application in the diagonostic, agglutination, precipitation, complement fixation, Neutralisation, RIA. e)                  Principle and Method of ELISA Tests. III. Collection and processing of faeces samples, concentration techniques of stool for Microscope Examination Parasitology - Morphology and Lab diagnosis of E-Histolytica, Gardia, Trichomonas, Plasmodium, Leshmania, Ankylostoma - deodenale, Ascaris Lumbricoidus, Taenia, E-Granulosus, Enterobius - Vermicularis, Dracunculus Medinensis, Wucharia Ban crofti IV.              Antiboitic sensitivity test - Preparation of Antibiotic discs V.                 Preservation Methods of Stock cultures and their importance and principle procedure. VI.              Brief outline of Morphology cultural characteristics and lab	1.                  Collection of clinical materials like blood, urine, stool, sputum, swabs etc. 2.                  Parasitology - collection, preservation and transportation of faecal material for examination of parasites. Concentration techniques of stool for ova and cyst. Wet preparation of faecal sample for ova and cyst. Identification of ova and cyst in stool sample. 3.                  Procedure of techniques of sputum for AFB. 4.                  Procedure of skin clipping of Leprae Bacilli. 5.                  Identification of organisms with Biochemical reactions of common organism like - staphylococus, E.coli - Klebsiella, shigella, Salmonilla, Proteus, Psudomonas. 6.                  Antibiotic Sensitivity tests 7.                  Preservation of stock culture 8.                  Bacteriology of water 9.                  Bacteriology of Milk 10.              Bacteriology of food Mycology 11.              Collection of specimen for fungal examination like skin scrapings, swabs, CSF. 12.              Fungal examination by wet preparation
diagnosis of imp. pathogens a)                  Gram Positive - staphylococus, Streptococus, Pneumococus b)                  Gram Negative - Gonococi, Meningococci c)                  Gram Possitive Bacilli - Coryn- Diptheria, Myco-tuberculosis, Mycro-leprae, B. anthracis d) Gram Negative Bacilli - Enterbacteriaceae - E.Coli, Klebsiella, salmonella, shigella, Enterobacter, proteous. e)                  Anarobic Bacterial, Bacteriodes, clostridium f)                   Vibriocholera - Pseudomonas g)                  H.influenza - B. Pertusis h)                  Spirochaetes - Treponema, leptospira, Borrelia i)                    Actinomyes & Nocardia VII.           Bacteriological examination and water, milk & food. VIII.        Mycology - Morphology cultural characteristics and lab diagnosis a) Candida b) Cryptococcus c) Dermatophyta d) Aspergillus e) Pencillium IX. Virology - Classification, General properties and cultivation and imp . pathogenic viruses such as Measles, Mumps, Influenza, polio, Hepatitis, Rabies, Herpes, Rubella, HIV, Dengue, and J.E.	13.              Fungal culture Serology 29 14.              VDRL Tests 15.              Preparation of widal Antegens & widal tests 16.              Brucella Agglutination test 17.              Weil felix test 18.              Paul Bunnel test 19.              RA test 20.              CRP test 21.              TPHA Virology 22.              ELISA test 23.              Western blot test 24.              Incubation of fertile eggs and innoculation by various routes 25.              Model layout of Animal House. 26.              Feeding, breeding and handling of Animals 27.              Techniques of drawing of blood from animals 28.              Anesthesia for animals 29.              Common diseases of the lab animals - and preventive aspects.
X.                 Vaccines - classification and uses XI.              Layout of Animal House XII.           General priciples of animal care - feeding, breeding and handling of animal and disposal of animal waste XIII.        Anaesthesia for animals, Euthanesia, Rethins, and frog 27 XIV.        Techniques of drawing a blood from animals XV.           Common disease of the lab animals preventive, aspects and disposal of animals and related Malerial

 

REFERENCE BOOKS

1.      Praful-Godkar - Text Book of Medical Lab Technology

2.      Ramnik Sood - Text Book of Medical Lab Technology

3.      K.M. Samuel - Manual for Medical Lab Technology

4.      Harold Varley - Practical Clinical Biochemistry

5.      Lehninger - Textbook of Biochemistry

6.      Rama Rao - Textbook of Biochemistry

7.      C.C. Chatterjee - Human Physiology

8.      Chowrasia - Human Anatomy

9.      Anantha Narayan - Text Book of Microbiology

10.  Toratora - Anatomy & Physiology

11.  Indesten Singh - Histology

12.  Chaurasia - Gross Anatomy

13.  WHO Lab Manual

 

 

 

List of Equipments Required to available in Institute

Biochemistry 1

1.             Hot Plate 1

2.             Gas Cyllinder with Burner 1

3.             Spirit lamps 15

4.             Hand Centrifuges 5

5.             Electrical Centrifuges 1

6.             Refrigerator 165 lit. 1

7.             Colorimeter 1

8.             Hot air oven 1

9.             Water bath 1

10.         Analytical Balance 5

11.         Physical Balance 2

12.         Typewriter

13.         Flame photometer 1

14.         Spectro Photometer

15.         Flourimeter 1

17.           PH Meter

18.           Incubator 1

19.           Electrophoresis apparatus 1

20.           Computer 1

21.           Semi auto analyser 1

Glassware

1.     Test tubes

18 x 150 mm - 100

15 x 150 mm - 100

15 x 125 mm - 100

2. Centrifuge tubes  36 

16 x 100 mm - 200

3.  Fol in sugar tube 30

4.  Beaker-glass and polypropylene 250 ml 5

 

5.      Pipettes a)        Volumetric-capacity 2 ml - 6 Nos 5 ml - 5 Nos 10 ml - 6 Nos 20 ml - 5 Nos 25 ml - 5 Nos b)       Serological blow out type 1  ml 1/100 - 10 nos. 2  ml 1/100 - 10 nos. 5 ml 1/100 - 10 nos. 1.10 ml 1/10ml - 5 nos. 2 ml 1/10 ml - 5 nos. 0.1  ml 1/100 ml - 5 nos. 0.2  ml 1/100 - 5 nos. c)        Ostwald pipettes 0.1 ml - 4 Nos. 0.2 ml - 4 nos. 0.5 ml - 4 6.       Burettes 25 ml - 4 50 ml – 4 7.       Reagent Bottles 60 ml - 10 nos. 120 ml - 10 nos. 250 ml - 20 nos. 500 ml - 5 nos. 100 ml - 5 8.       Dropper bottles 30 ml 5 nos.

9.           Watch glass (Assorted sizes) - 6 Nos 10.       Volumetric Flasks 25 ml - 6 nos. 50  ml - 6 nos. 100 ml - 10 nos. 250 ml - 10 nos. 500 ml - 10 nos. 1000 ml - 5 nos. 11.       Stoppered graduated Test Tubes 15 ml - 10 nos. 40 ml - 10 nos. 50  ml - 10 nos. 12.       Distillation assembly (glass) Complete set 1 13.       Condensor 1 14.       Round Bottom flask 500 ml - 1 no. 1000 ml - 1 no. 15.       Filter Paper - Ordinary - 1 Ream 16.       Whatman Filter Paper 46x57 cm No 1 - 20 sheet No 2 - 10 sheets 17.       Cotton (absorbant) 5 Rolls 18.       Glass slides 5 boxes 19.       Plastic Wash bottles 50 ml. 10 nos. 20.       Mortar/Pestle - 2 nos.

 

 

Microbiology 1.             Student Microscope - 5 2.             Centrifuge - 2 3.             Refrigerator - 1 4.             Autoclave - 1 5.             Hot air oven - 1 6.             Incubator - 1 7.             Inspissator - 1 8.             Deioniser - 1 9.             Distil water plant - 1 10.         Pipette washer - 1 11.         Anaerobic Jar - 1 12.         Vaccum Pump - 1 13.         Analytical Balance - 1 14.         Water Bath - 1 15.         VDRL Rotator - 1 16.         Electrophoresis apparatus - 1 17.         Petri dishes - 100 x 17 - 100 nos 18.         Test Tubers - 150 x 19 -100 100 x 12 -100 19.         Pipettes 10 ml - 10 nos. 5 ml - 10 nos. 1 ml - 10 nos. 20.         Wash bottles - 5 21.         Spatulas - 12 22.         Reagent bottles - 10 23.         Measuring Cylinders 50 ml - 5

 

Pathology 1.             Microscope 1 2.             Hot air oven 1 3.             Incubator 1 4.             Centrifuge 1 5.             Blood cell counter 2 6.             Water Bath 1 7.             Chemical Balance 1 8.             Hot plate 1 9.             Stopwatch 1 10.         Haemometer 5 11.         Haemocytometer 5 12.         ESR stand 5 13.         ESR Tubes 5 14.         Mortor and Pestle 2 15.         Urinometer 2 16.         Spiritlamp 2 17.         Syringe 20 ml - 10 10 ml - 10 5 ml - 5 2 ml - 2 18.         Beaker 100 ml - 5 250 ml - 5 A.Collaborating Institutions for Curriculum transaction 1.         All Hospitals 2.         All Medical Colleges 3.         All the national laboratories 4.         Regional Research Laboratories 5.         University Departments 6.         Pharmaceutical Companies and Education Institutes B. On the Job Training Centres 1.           Government Head Quarters Hospital 2.           PHCs 3.           Dispensaries 4.           Medical colleges 5.           Private Hospitals 6.           Private labs

 

 

IX. Qualification of Lecturers

1.  MBBS

2.  MBS Hom/BHMS

3.  B. Pharmacy

4.  M.Sc. Microbiology/Biochemistry/MLT

X. Vertical Mobilities

A) With Bridge Course

1)  B.SC (BZC)

2)  Courses through EAMCET

B) Without Bridge Course

1)  B.Sc. MLT

2)  B.Sc. Microbiology

3)  B.Sc. Biochemistry

4)  B.Sc. Biotechnology

5)  M.Sc. MLT/Biochemistry/Microbiology/Biotechnology (at P.G. level)